Phytochemical analysis of Buchanania axillaris leaves extract
Abstract
The present study carried out on phytochemical analysis of Buchanania axillaris leaf extract. The extraction was done with sohlet extraction method using methanol. The methnol extract was analyzed for its phytochemical constituents using standard test procedures and depends on results; thin layer chromatography was studied using different mobile phases. Then the extract used for isolation of bioactive molecules using column chromatography. The results of the phytochemical analysis says that, methanolic extract of B. axillaris leaves posses different phytochemicals like steroids, phenolics, flavanoids etc., and on chromatographic isolation, three compounds were isolated (BAL-01, 02, 03). The isolated compounds were identified as beta-sitosterol, quercetin, kamepferol based on structural elucidation studies (IR, MP, and NMR). The isolated compounds are first time reporting from the B. axillaris.
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