Simultaneous estimation of emtricitabine, tenofovirdisoproxilfumarate and rilpivirine in pharmaceutical dosage form by using rp-hplc

  • Kanchan Sunil Nautiyal Gitam University, Goregaon West, Mumbai, Maharashtra - 400104, India
  • G. Indira Priyadarshini Hindu College of Pharmacy, Amaravathi Road, Guntur – 522002, A.P., India
  • P. Sukhanya Hindu College of Pharmacy, Amaravathi Road, Guntur – 522002, A.P., India

Abstract

The study describes method development and subsequent validation of RP-HPLC method for simultaneous estimation of emtricitabine, tenofovir disoproxil fumarate and rilpivirine in combined tablet dosage forms. Chromatographic separation was achieved on a hypersilBDSC18 column (250 mm x 4.6 mm, 5 µm) using a mobile phase consisting of (45:55 v/v) buffer: acetonitrile at a flow rate of 1 mL/min. The detection wavelength is 280 nm. The retention times of emtricitabine, tenofovirdisoproxil fumarate and rilpivirine were found to be 2.692, 4.402 min and 5.725 min respectively. The developed method was validated as per ICH guidelines. The developed and validated method was successfully used for the quantitative analysis of emtricitabine,tenofovirdisoproxilfumarate and rilpivirine in tablet dosage forms.

Keywords: HPLC, emtricitabine, tenofovirdisoproxilfumarate, rilpivirine, Validation

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How to Cite
Nautiyal, K. S., G, I. P., & P, S. (2023). Simultaneous estimation of emtricitabine, tenofovirdisoproxilfumarate and rilpivirine in pharmaceutical dosage form by using rp-hplc. Journal of Integral Sciences, 6(4), 13-20. https://doi.org/10.37022/jis.v6i4.66
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Research Article(s)