Genetic diversity and Molecular characterization of few citrus species in Visakhapatnam by RAPD markers

  • Srilekha V MicGene Laboratory, Visakhapatnam, Andhra Pradesh-530003.
  • Ravi Sankar U MicGene Laboratory, Visakhapatnam, Andhra Pradesh-530003

Abstract

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and interrelationship among 5 citrus species, including C. aurantium, C. sinensis, C. lemon, C. aurantifolia and C. trifoliata. Out of 20 decamer primers screened, 5 were selected which produced reproducible amplification; in that 54 bands were observed, of which 46 were polymorphic. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated every species distinguishably. The maximum similarity was observed to be seen between C. aurantium and C. sinensis of approximately, 0.605 and the least between C. aurantifolia and C. lemon of approximately 0.350. And same phenomenon was observed in distance matrix also.

Keywords: Citrus, Random Amplified Polymorphic DNA, Genetic diversity, Similarity

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V, S., & Sankar U, R. (2018). Genetic diversity and Molecular characterization of few citrus species in Visakhapatnam by RAPD markers. Journal of Integral Sciences, 1(3), 22-27. Retrieved from https://jisciences.com/index.php/journal/article/view/19
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Research Article(s)