Genetic diversity and Molecular characterization of few citrus species in Visakhapatnam by RAPD markers

  • Srilekha V MicGene Laboratory, Visakhapatnam, Andhra Pradesh-530003.
  • Ravi Sankar U MicGene Laboratory, Visakhapatnam, Andhra Pradesh-530003


Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and interrelationship among 5 citrus species, including C. aurantium, C. sinensis, C. lemon, C. aurantifolia and C. trifoliata. Out of 20 decamer primers screened, 5 were selected which produced reproducible amplification; in that 54 bands were observed, of which 46 were polymorphic. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated every species distinguishably. The maximum similarity was observed to be seen between C. aurantium and C. sinensis of approximately, 0.605 and the least between C. aurantifolia and C. lemon of approximately 0.350. And same phenomenon was observed in distance matrix also.

Keywords: Citrus, Random Amplified Polymorphic DNA, Genetic diversity, Similarity


Abkenar A, Isshiki S. 2003. Molecular characterization and genetic diversity among Japanese acid Citrus (Citrus spp.) based on RAPD markers. J Hort Sci Biotech 78, 553–556.
Aseel K, AL-Anbari, Nantawan Kanawapee, Talib A, AL-Kazragi et al., 2014. Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers. Afr J Agric Res 9(11), 1012–1019.
Bayer RJ, Mabberley DJ, Morton C, Miller CH, Sharma IK et al., 2009. A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. Am J Bot 96(3), 668–685.
Citrus fruit statistics. 2015. Food and Agriculture Organization of The United Nations, Rome, 2016.
CITRUS (Citrus spp) - drysrhu. 2017.
Coletta Filho HD, Machado MA, Targon MLPN, Moreira MCPQDG, Pompeu J Jr. 1998. Analysis of phylogenetic diversity among mandarins (Citrus spp.) using RAPD markers. Euphytica 102:133–139.
Federici CT, Fang DQ, Scora RW, Roose ML. 1998. Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor Appl Genet 96, 812–822.
Fernandez ME, Figueiras AM, Benito C. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphisms, genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet 104, 845–851.
Hodgson RW. 1967. Horticultural varieties of citrus. In: Reuther, W., Webber, H.J. and Batchelor, L.D. Eds. The Citrus Industry, Vol. I. University of California Press, Berkeley, 431–592.
Inga G, Tamara K, Boris V, Marina K, Nelly D. 2014. Application of PCR-based methods for rapid detection of corn ingredients in processed foods. Int J Nutr Food Sci 3, 199–20.
Luro F, Gatto J, Costantino G, Pailly O. 2011. Analysis of genetic diversity in Citrus. Plant Genet Resourc 9(2), 218–221.
Malik SK, Rohini MR, Susheel Kumar, Ravish Choudhary, Digvender Pal et al., 2012. Assessment of Genetic Diversity in Sweet Orange [Citrus sinensis (L.) Osbeck] Cultivars of India Using Morphological and RAPD Markers. Agric Res 1(4), 317–324.
Maya MA, Rabbani MG, Mahboob MG, Matsubara Y. 2012. Assessment of genetic relationship among 15 Citrus fruits using RAPD. Asian J Biotech 4, 30–37.
Moore GA. 2001. Oranges and lemons: clues to the taxonomy of Citrus from molecular markers. Trends Genet 17, 536–540.
Natividade Targon MLP, Machado MA, Coletta Filho HD, Cristofani M. 2000. Genetic polymorphism of sweet orange(Citrus sinensis [L.] Osbeck) varieties evaluated by random amplified polymorphic DNA. Acta Hort 535, 51–53.
Nicolosi E, Deng ZN, Gentil A, Lamalfa S, Continella G et al., 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100, 1155–1166.
Novelli VM, Cristofani M, Machado MA. 2000. Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis (L.) Osbeck. Acta Hort 535, 47–49.
Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O. 2009. Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scientia horticulturae 121(3), 306-312.
Virk PS, Zhu J, Newburg HJ, Bryan GJ, Jeckson MT et al., 2001. Effectiveness of different classes of molecular markers for classifying and revealing variation in rice germplasm. Euphytica 112, 275-284.
Weising K, Nybom H, Wolff K, Kahl G. 2005. DNA fingerprinting in plants’ principles, methods and applications. 2nd edn. Taylor & Francis, Boca Raton.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18, 6531–653
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V, S., & Sankar U, R. (2018). Genetic diversity and Molecular characterization of few citrus species in Visakhapatnam by RAPD markers. Journal of Integral Sciences, 1(3), 22-27. Retrieved from
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